Arrayed CRISPR screening for phenotypic discovery

The Functional Genomics Screening Laboratory (FGSL) leverages the unique features of arrayed screening, whereby individual genes are edited via CRISPR in plate-based format, to uncover the complexity of developmental and disease signatures using human cellular models. The laboratory is equipped with a high-throughput screening platform to enable functional endpoint acquisition including high content imaging and flow cytometry.

This presentation will showcase previous phenotypic screens in complex cell models, in which we overcame some of the inherent challenges in delivering CRISPR/Cas9 reagents. We will also present routes to form either academic or small/medium-sized enterprise collaborations with the FGSL for arrayed screening.

Key Topics Include:

• CRISPR screening as an unbiased approach to identify new targets for hypothesis generation.
• Arrayed format facilitates dissection of complex phenotypes.
• Non-viral delivery of CRISPR/Cas9 reagents such as through nucleofection enables arrayed screening in primary cells.